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KMID : 0545119970070050333
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 5 p.333 ~ p.340
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Selection and Identification of a Strain KT-10 Producing the Cathepsin B Inhibitor
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Han, Kil Hwan
Do, Jae Ho/Kim, Sang Dal
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Abstract
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An actinomycetes, KT-10 isolated from ginseng field in Kyongpook, Korea was selected based on its ability to produce a lysosomal cathepsin B inhibitor. The inhibitor purified from the culture supernatant of the isolate KT-10 showed strong inhibitory effects against cathepsin B as well as against papain when the activities were measured using synthetic substrate, α-N-benzyloxycarbonyl-L-lysine p-nitrophenyl ester (CLN) or α-N-benzoyl-D,L-arginine 2-naphthylamide (BANA). The isolate KT-10 was identified as a species of Streptomyces based on its morphological characteristics and chemotaxonomic data. The TAXON program of Ward was used to identify Streptomyces sp. KT-10 as a strain of Streptomyces luteogriseus belong to cluster 18 of the genus Streptomyces with a Willcox probability 0.999388. The cathepsin B inhibitor was presumed to a novel material composed of a polyhydroxylamine.
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